Fig. 5
In vitro anti-metastasis and anti-tumor effects of IGBN. a Representative wound-healing assay to track cell migration at 0 h and 24 h. Pla: platelets. E2: 17β-estradiol. Scale bar: 200 µm. b Quantification of cell migration by Image J (n = 6). c Representative images of migration and invasion in 4T1 tumor cells treated with different formulations in the Transwell assay. Scale bar: 200 μm. d and e Corresponding data analysis of the transwell assay by Image J (n = 6). f Fluorescence images of calcein AM (green, live cells) and PI (red, dead cells) co-stained 4T1 cells after incubation with different treatments for 24 h. Scale bar: 200 μm. g Flow cytometer analysis of apoptosis in 4T1 cells after incubation with different treatments for 24 h. Results are presented as means ± s.d. Statistical significance was calculated by Student’s t-test. ***P < 0.001, ****P < 0.0001