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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Huc-MSCs-derived exosomes attenuate inflammatory pain by regulating microglia pyroptosis and autophagy via the miR-146a-5p/TRAF6 axis

Fig. 3

Huc-MSCs-derived exosomes inhibited BV2 cells pyroptosis. a BV2 cells were administered LPS (1 µg/ml) for 1 h, 12 h, 24 h and ATP (5 mM) for 30 min, and NLRP3, GSDND, ASC and caspase-1-p20 were tested by western blot at the indicated time points. b CCK-8 assay was used to assess the effect of huc-MSCs-derived exosomes (1, 10, 50, 100 µg/ml) on BV2 cell viability, and ns indicates no significance. c Compared with con group, the protein levels of NLRP3, GSDMD-F, and GSDMD-N were decreased in BV2 cells pretreated with 10 and 100 µg/ml exosomes. d ELISA was used to test IL-1β in supernatants from different groups. e Different groups of BV2 cells were stained with Yo-Pro-1 (green), which is membrane impermeable but pyroptosis-pole permeable dye. Hoechst (blue) was used to stain nucleus. BV2 cells treated with 0.1% Triton were used to be the positive control group, bar = 50 µm. Data are presented as the mean ± standard deviation (SD). (***P < 0.001)

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