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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Huc-MSCs-derived exosomes attenuate inflammatory pain by regulating microglia pyroptosis and autophagy via the miR-146a-5p/TRAF6 axis

Fig. 5

Huc-MSCs-derived exosomes inhibited pyroptosis and increased autophagy via transferring miR-146a-5p to BV2 cells. a Top ten miRNAs in GSE159814 and GSE69909. b RT-PCR was used to assay the relative levels of miR-146a-5p in BV2 cells treated with huc-MSCs-derived exosomes or PBS. c Four different miRNA target databases, miRWalk, miRBD, TargetScan, ENCORI, were used to predict the potential target genes of miR-146a-5p. d The protein levels of TRAF6, NLRP3, GSDMD-F, GSDMD-N, ASC and caspase1-p20 were measured by western blot in BV2 cells pretreated with miR-146a-5p inhibitor exosomes or miR-146a-5p mimic exosomes. e Autophagy-related proteins, P62 and LC3, were tested by western blot in BV2 cells pretreated with miR-146a-5p inhibitor exosomes or miR-146a-5p mimic exosomes. Data are presented as the mean ± standard deviation (SD). (**P < 0.01)

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