Fig. 6From: Huc-MSCs-derived exosomes attenuate inflammatory pain by regulating microglia pyroptosis and autophagy via the miR-146a-5p/TRAF6 axisTRAF6 knockdown inhibited LPS and ATP induced BV2 cell pyroptosis and increased autophagy. a BV2 cells were administered LPS (1 µg/ml) for 0 h, 1 h, 6 h, 12 h, 24 h and ATP (5 mM) for 30 min, and TRAF6 was tested by western blot at the indicated time points. b Western blot analysis of NLRP3, TRAF6, GSDMD-F and GSDMD-N in BV2 cells transfected with siRNA targeting TRAF6 for 48 h and then stimulated with LPS (1 µg/mL) and ATP (5 mM) for 30 min. c BV2 cells pretreated with si-TRAF6, LPS, ATP were stained with Yo-Pro-1 and Hoechst, bar = 50 µm. d Levels of P62 and LC3 protein were measured by western blot in BV2 cells transfected with si-TRAF6. e, f Autophagy was visually observed via mCherry-GFP-LC3 adenovirus transfecting BV2 cells and quantitation of autolysosomes. Data are presented as the mean ± standard deviation (SD). (**P < 0.01, ***P < 0.001)Back to article page