Fig. 7

Huc-MSCs-derived exosomes inhibited pyroptosis through promoting autophagy via transferring miR-146a-5p in vivo. a The mRNA levels of miR-146a-5p in different groups were measured by RT-PCR. b Expression level of TRAF6 protein in the spinal cord measured by western blotting. c, d Huc-MSCs-derived exosomes decreased the expression of NLRP3 and pyroptosis-related proteins in the spinal cord. e, f Expression levels of LC3, beclin1, and P62 were measured in mice intrathecally injected with huc-MSCs-derived exosomes. g Mice were administered vehicle or 3-MA, RAP via intrathecal injection 1 h before modelling, and NLRP3, GSDMD-F, GSDMD-N, and Caspase1-p20 proteins were tested by western blot. Data are presented as the mean ± standard deviation (SD). (*P < 0.05, **P < 0.01, ***P < 0.001)