Fig. 5
a XFM images of MIA PaCa-2 cells treated with bTiO2-Gd-COOH, bTiO2-Gd-IGF1 or bTiO2-Gd-IGF1-GEM. Biogenic chlorine element is depicted as red-bright, and Ti element of nanoprobes is depicted as yellow-bright. Scale bar is 10 μm. b ICP quantitative analysis, and c Confocal microscope images of nanoprobes incubated MIA PaCa-2 cells. Blue fluorescence is nucleus labeled with Hoechst 33342, green fluorescence is cell membrane stained with FITC-phalloidin, and red fluorescence is rhodamine stained nanoprobes. Scale bar is 50 μm