Fig. 1From: Protective role of small extracellular vesicles derived from HUVECs treated with AGEs in diabetic vascular calcificationIdentification of EC/sEVs. Small extracellular vesicles (sEVs) derived from HUVECs with or without AGEs pre-treatment (A-EC/sEVs, EC/sEVs). A Representative image of the ultrastructure of sEVs observed by transmission electron microscopy. B The average particle size distribution of sEVs using nanoparticle tracking analysis. C Analysis of the possible contaminants BSA and the sEVs markers CD9, CD81 and TSG101 by western blotting in sEVs preparations. The conditioned media after sEV removal was used as a control. D The expression of sEVs markers after proteinase K (PK) or Triton X-100 treatment detected by western blottingBack to article page