Fig. 5

Highly expression or phosphorylated activation of GRP75 promotes centrosome duplication. (A, G) Confocal imaging the co-localization of GRP75 with γ-tubulin in asynchronously and synchronously growing Skov3 cells. Arrows point to the positions of centrosomes. Images placed on the up-right show the magnification of areas indicated by arrows. Scale bar, 10 μm. (B–F, H–K) The frequencies of GRP75 co-localization with γ-tubulin among unduplicated and duplicated centrosomes were determined and plotted, respectively. (L) Schematic centrosome reduplication assay. (M) Confocal imaging of replicated and non-replicated centrosomes in hydroxyurea (HU)-exposed cells, and (N) cell-cycle was determined by flow cytometry analysis. (O, P) The frequencies of centrosomes per cell were scored and shown in asynchronously and synchronously growing Skov3 cells with GRP75-KD or -OE. 60 cells were counted for any sub-phase or cell line. Average values are shown