Fig. 6

Highly expression or phosphorylated activation of GRP75 promotes itself and Mps1 translocating to centrosome. (A) Confocal imaging the co-localization of MPS1 and γ-tubulin, GRP75 and γ-tubulin, GRP75 and γ-tubulin in Skov3 cells with GRP75-KD or -OE. (B–F) Fluorescent intensities along the central line of centrosome were plotted, and staining signal distribution curves of GRP75, MPS1 and γ-tubulin were determined by ImageJ software and are shown. (G) Centrosome extracts prepared from Skov3 cells were subjected to sucrose gradient centrifugation, and the fractions were western blotted to identify the fraction enriched for centrosomes. (H) Western blot determined the centrosome-resident level of GRP75 and MPS1 in Skov3 cells with GRP75-KD or -OE