Fig. 1
From: A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses
The extraction and identification of polysaccharide. a The extraction process and chemical structure of PRBS. b-g Expansions of the NMR spectra of PRBS. The 1H NMR b and 13C NMR c of 50Â mg PRBS dissolved in D2O by NMR of 800Â MHz. Glucose and mannose were identified in PRBS sample. d Heteronuclear single quantum correlation provided the correlations between a carbon and its attached protons. e The heteronuclear multiple-bond correlation (HMBC) provided the correlations to carbons, including non-protonated carbons, two or three bonds away from the proton. f 1H/1H chemical-shift correlation spectroscopy (1H-1H-COSY) provided proton-proton correlations g The 1H/1H-total correlation spectroscopy (1H/1H-TOCOSY) provided the correlations from a proton to other protons within the same proton spin system