Fig. 4

Validation of the biomarker panel identified by random forest model. a OPLS-DA plots of EVs lipids of two groups based on the selected 4 markers: FFA (18:0), LPC (22:6/0:0), FFA (18:1), PI (16:0/18:1). b ROC curves constructed by selected marker panel in the training set and testing set. c The diagnostic potency of the individual markers. d–g The relative abundance of each marker in the NAFL and NASH group for the training set and (h–k) testing set