Fig. 9
From: Iron oxide and iron oxyhydroxide nanoparticles impair SARS-CoV-2 infection of cultured cells
FAC, Venofer and IONP treatments alter the iron metabolism in Vero E6 cells. A The effect of treatment with FAC, Venofer or IONPs on the expression of genes involved in iron metabolism. B The effect of SARS-CoV-2 infection on genes involved in iron metabolism. C The concentration of intracellular iron in non-infected and SARS-CoV-2-infected cells was measured by ICP-OES. D The effect of FAC, Venofer or IONP treatment on genes involved in iron metabolism in SARS-CoV-2-infected cells. Confluent monolayers of Vero E6 cells were infected with SARS-CoV-2 (MOI, 0.001) and at 1 hpi, the extracellular medium containing the virus was replaced with a suspension of FAC, Venofer or IONPs . Total RNA was purified at 24 hpi, and the expression of SLC11A2, SLC40A1, SLC48A1, TFRC, LCN2 and IREB2 was analyzed by qRT-PCR and normalized to the GAPDH expression in each sample. Data shown as the mean ± SD (n = 5)