Fig. 3

Hydrophobicity and zeta potential effect on NP adsorption to human RBCs. a Coupling efficiency of PLGA65:35 and PLGA50:50 NPs onto human RBCs at different RBC:NP incubation ratios. b Percentages of human RBC populations carrying PLGA65:35 or PLGA50:50 NPs. c Hemolysis percentages of human RBCs after incubation with PLGA65:35 or PLGA50:50 NPs at different RBC:NP incubation ratios. Hemolysis percentages are represented as total hemolysis subtracted by hemolysis of control cells. d Coupling efficiency of PLGA65:35 and PEI-PLGA65:35 nanoparticles onto human RBCs at different RBC:NP incubation ratios. e Percentages of human RBC populations carrying PLGA65:35 or PEI-PLGA65:35 NPs. f Hemolysis percentages of human RBCs after incubation with PLGA65:35 or PEI-PLGA65:35 NPs at different RBC:NP incubation ratios. Hemolysis percentages are represented as total hemolysis subtracted by hemolysis of control cells. g Agglutination assay of human RBCs with PLGA65:35 or PLGA50:50 NPs or PEI-PLGA65:35 NPs at different RBC:NP incubation ratios (n = 3). All data are presented as mean ± SEM (n = 3). The number of asterisks indicate the level of significance (two-way ANOVA with Sidak multiple testing adjustment), where: *p < 0.05, **p < 0.01 and ***p < 0.001