Fig. 4From: Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathwaysThe effect of electrospun membranes on wound healing in skin defects in mice. A Skin defect healing in mice at different time points. B Line diagram showing skin defect healing in mice at different time points. * indicates a significant difference between the A20 and Con groups, # indicates a significant difference between the A20 and R20 groups. C Statistical analysis of the remaining wound area in the three groups on day 14. (**p < 0.01, ##p < 0.01, ***p < 0.001, n = 5). D, E Immunofluorescence staining of CD86 and CD206 in each group of skin sections on day 7. F, G Mean fluorescence intensity of CD86 and CD206 in each group of skin sections on day 7. H, I Immunofluorescence staining of CD86 and CD206 in each group of skin sections on day 14. J, K Mean fluorescence intensity of CD86 and CD206 in each group of skin sections on day 14. DAPI (blue) was used to label the nucleus, F4/80 (green) was used to label macrophages, and CD86 or CD206 (red) was used to label M1- or M2-phenotype macrophages, respectively (*p < 0.05, **p < 0.01, *** p < 0.001, n = 3)Back to article page