Fig. 4

In vitro effects of PCL/VMT composite scaffolds on wound healing-related cell behavior. A In vitro tube formation assay of MAECs. The quantitative analysis of the number of nodes (B) and the tube length (C) formed on the Matrigel. D The angiogenesis-related gene expressions of HIF-1α, VEGF, SDF-1α, eNOS, Flt-1, KDR, and Tie-2 in MAECs. E Immunofluorescence staining of CD31, Phalloidin, and DAPI in MAECs (red: positive area of CD31; green: cytoskeleton; blue: cell nuclei). F The gene expressions of COL I, COL III, FN, and bFGF in L929 cells. Data represent means ± SD (n = 3), n.s. (not significant), *P < 0.001, **P < 0.01, ***P < 0.001, ****P < 0.001