Fig. 2

Local gel scaffold for eliciting immunogenic tumor phenotypes. 4T1-Luc tumors harvested from mice implanted with empty hydrogels, DOX@Gel, JQ1@Gel or DOX-JQ1@Gel were analyzed by flow cytometry 2 days after treatment. A Corresponding quantification analysis of T cell infiltration within the tumor. B Corresponding quantification analysis of CD8⁺ T cell infiltration within the tumor. C Corresponding quantification analysis of CD4⁺ T cell infiltration within the tumor. D Corresponding quantification analysis of MDSCs (CD11b⁺Gr-1⁺), gating on CD45⁺ cells. E Corresponding quantification analysis of M2 macrophages (CD206⁺) in F4/80⁺ CD11b⁺ CD45⁺ cells. F Corresponding quantification analysis of regulatory T cells (Tregs, FOXP3⁺ in CD4⁺ CD25⁺ CD45⁺ cells). G PD-L1 expression of tumor cells after empty hydrogel or DOX-JQ1@Gel treatment. H PD-1 expression of CD4⁺ and CD8⁺ T cell after empty hydrogel or DOX-JQ1@Gel treatment