Fig. 3

H22 and T cell dual cellular uptake and gene silencing. CLSM images of a T cells and b H22 tumor cells treated with CS@P in media at pH 7.4 or 6.5, respectively. Scale bar: 50 µm. Flow cytometry images of cellular uptake of FITC-labeled nanoparticles by c T cells and d H22 tumor cells at pH 7.4 and pH 6.5 conditions for 4 h. e Western blot analysis of the indicated proteins in T cells (left) and H22 tumor cells (right) 48 h after the incubated with CS. f, g Transwell results of the effect of NR2F6 on migration (left) and invasion (right). h CLSM images of PD-L1 expression in H22 cell after the indicated treatments. i Cytokine levels in the supernatant on 48 h after the indicated treatments (n = 3)