Fig. 6

Abscopal effect of CS@P combined with NIR irradiation and aPD-L1. a Distant tumor growth tendency of H22 tumor-bearing mice with various treatments. Tumor sizes were normalized to initial sizes. Error bars stand for ± SD (n = 6). b Distant tumor growth curves in the H22 tumor-bearing BALB/c mice (n = 6). c Images of the distant tumor harvested on day 21. d The amount of CD4⁺ and CD8⁺ T cells in distant tumors detected upon various treatments detected by flow cytometry (gated on the CD3⁺). Data represented mean ± SD (n = 3). e The amount of Treg cells in distant tumors upon various treatments detected by flow cytometry Data represent mean ± SD (n = 3). f Frequency of the CD4⁺ and CD8⁺ T cells in spleens after indicated treatments. g Frequency of the Treg cells in spleens after indicated treatments. The levels of IFN-γ (h), TNF-α (i), and IL-2 (j) in the primary tumor after the various treatments (n = 3), expressed as the concentration per gram of tumor (pg/per g tumor). *P < 0.05, **P < 0.01, and ***P < 0.001