Fig. 1

 A Characterization of starting orbit via PAGE. The concentration of target and S was 1 µM. The concentration of Q was 500 nM. B Characterization of amplification orbit via fluorescence. The concentration of Ra and Rb probe was 50 nM. The concentration of S’ was 100 nM. C Comparison of RHOA amplification efficiency. Schematic Illustration of the amplification process of RHOA, non-local RHOA and the classic enzyme-free DNA amplification technology-entropy-driven amplification reaction (EDAR). a Fluorescence kinetic curves of RHOA and non-local RHOA, k₁: the amplification rate of RHOA, k₂: the rate of non-local RHOA. b Fluorescence kinetic curves of RHOA and EDAR, k₁: the amplification rate of RHOA, k₃: the rate of EDAR. D Schematic drawing of comparison of the reaction area and local concentration of localized RHOA and non- localized RHOA