Fig. 4
Evaluation of the analyze performance of the ECL biosensor. A ECL signal responding to 10− 2, 10− 1, 1, 10, 102, 103, 104, 105, 106, 107, 108 fM of exo-miRNA-15a-5p (from a to k). B Linear correlation between the ECL intensity and the logarithm of target concentrations ranging from 10− 2 fM to 108 fM. C Evaluation of the stability of the ECL biosensor. D Verification of the specificity of the ECL biosensor. The current ECL signal respond to blank, miRNA-21, miRNA-101, miRNA-378, miRNA-155, miRNA-122, miRNA-15a-5p and mixture. E Evaluation of the intra-batch repeatability of purposed ECL biosensor. F Evaluation of the inter-batch repeatability of purposed ECL biosensor. All results expressed as mean ± standard variation (n = 3)