Fig. 10

Induced autophagy of breast cancer cell. A TEM results of MDA-MB-231cells after 20 µM TPP-SS-ATS-LS treatment. (i) Control group, (ii) and (iii) TPP-SS-ATS-LS group. The mitochondria were shown by the yellow arrows; the autophagy lysosomes shown in red arrows, the myelin-like structures shown by the blue arrows. Local organelles self-melt and degrade as shown by the green arrows, and individual lipids are shown by the purple arrows. The protein expression level was analyzed in MDA-MB-231 cells after TPP-SS-ATS-LS treatment at 0, 2.5, 5, 10 and 20 µM for 48 h by Western blot analysis. TPP-SS-ATS-LS down-regulated (B) PHB2 protein level and induced (C) LC3-II and (D) PINK1 protein expression increased in breast cancer cells. Data are presented as the mean ± SD of at least three independent experiments. Compared with Control group, *P < 0.05