Fig. 9
From: Development of artesunate intelligent prodrug liposomes based on mitochondrial targeting strategy
Effect of TPP-SS-ATS-LS on oxygen consumption rate (OCR) of breast MDA-MB-231 cell. A A schematic overview of the mitochondrial stress test. The black arrows indicate the subsequent addition of the ATPase inhibitor oligomycin, the uncoupling reagent FCCP, and the inhibitors of the electron transport chain rotenone/antimycin A. B MDA-MB-231 cells were seeded in a 96-well Seahorse cell culture plate overnight (mean ± SD, n = 3). Incubation with blank-LS, ATS, TPP-SS-ATS or TPP-SS-ATS-LS at concentration of 20 µM for 24 h (C–F). These are respectively representing individual parameters for (C) basal respiration. D Spare respiration, (E) proton Leak and (F) ATP linked respiration. Compared with Control group, *P < 0.05, **P < 0.01. Compared with ATS group, #P < 0.05, ##P < 0.01