Fig. 10
From: Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing
Reprinted (adapted) with permission from [166]. Copyright 2022 American Chemical Society. B Hypoxia-responsive CRISPR-Cas9 delivery nanoformulations. Design of the hypoxia-responsive CRISPR-Cas9 system to reduce tumor thermal tolerance, and hypoxia-triggered delivery of Cas9-sgHSP90α to the nucleus for gene editing and mild-photothermal therapy carried out with NIR light. Reproduced with permission [169]. C MicroRNA-responsive CRISPR-Cas9 delivery nanoformulations. Schematic diagram of microRNA-responsive DNA nanoflower for release of Cas9/sgRNA and enhanced genome editing. Reproduced with permission [176]
AÂ ATP-responsive CRISPR-Cas9 delivery nanoformulations. Schematic illustration of the self-assembly of ZIF-90/protein nanoparticle and ATP-triggered protein release from ZIF-90 nanoparticle inside cells.