Fig. 2
From: Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing
Reprinted (adapted) with permission from [98]. Copyright 2022 American Chemical Society. BÂ ZIF-8-based nanocarrier for the delivery of CRISPR-Cas9 plasmids: preparation of CRISPR-Cas9 plasmid-loaded nanostructures, intracellular delivery of the two kinds of plasmids to the nucleus for paxillin gene knock-in. Reproduced from Ref. [99] with permission from the Royal Society of Chemistry
Imidazole-based pH-responsive CRISPR-Cas9 delivery nanoformulations. AÂ Encapsulation of the negatively charged Cas9/sgRNA within positively charged ZIF-8 to form CC-ZIFs, endosomal escape of CC-ZIFs, and laser scanning confocal microscope images of cells before and after treatment with CC-ZIFs.