Fig. 3
From: Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing
PEI-based pH-responsive CRISPR-Cas9 delivery nanoformulations. A Preparation of AuNP/crRNA/Cpf1/PEI/ssDNA, and laser scanning confocal microscope images of crRNA and ssDNA colocalization in a primary human CD34+ HSPC after incubation with AuNP/CRISPR. Reproduced with permission [106, 109]. B Design of the DNA NC–based charge reversal assembly for CRISPR-Cas12a delivery: preparation of the assembly of Cas12a/crRNA/NC/PEI/Gal-PEI-DM, and the chemical structure of Gal-PEI-DM and mechanism for acidic environment triggered charge reversal. Reproduced with permission [108]