Fig. 4
From: Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing
Other cationic polymer-based pH-responsive CRISPR-Cas9 delivery nanoformulations. AÂ Design and preparation of NPs by pH-sensitive mPEG-PC7A polymer via self-assembly, loading either Cas9 RNP alone or both Cas9 RNP and single-strand oligonucleotides (ssODN, a donor DNA), as well as behaviors of NPs including endocytosis, endosomal escape, payload releasing in cytoplasm and delivery into nucleus. Reproduced with permission [110]. BÂ Structure of CRISPR-Gold. Reproduced with permission [111]