Fig. 6
From: Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing
GSH-responsive CRISPR-Cas9 delivery nanoformulations. Schematic illustrations of heterogeneous surface charge of streptococcus pyogenes Cas9 (SpCas9), formation of the covalently crosslinked NC for the delivery of the Cas9 RNP complex prepared by in situ free-radical polymerization, and depiction of the proposed mechanism of the cellular uptake of NCs and the subcellular release of the RNP. Reproduced with permission [127]