Fig. 5
Comparative transfection efficiencies of lPEI/pDNA and bPEI/pDNA complexes delivered upon mechanical cell stimulation. a Workflow of the post-delivery vibropolyfection experimentation. Twenty-four hours post-seeding, L929 cells were stimulated for 5 min at fin = 1,000 Hz, challenged with polyplexes, then grown for 24 hrs in standard culture conditions. Twenty-four hours after the addition of polyplexes to the cells, cytotoxicity and Gaussia luciferase activity were assessed. b Fold increase in Gaussia luciferase activity evaluated on L929 cells transfected with lPEI/pGLuc and bPEI/pGLuc following pre-delivery and post-delivery vibropolyfection protocols. c Fold increase in Gaussia luciferase activity evaluated on L929 cells transfected according to the protocol described in a at different periods (i.e., 0 hr, 1 hr, 2 hrs, and 4 hrs) from the discontinuation of the mechanical stimulation. Each number within bars in the panels b and c refers to the average fold-increase value of the dataset. Results are expressed as mean ± SD (n ≥ 3)