Fig. 2

Isolation and characterization of neutrophils from human peripheral blood. a The morphological characteristics of neutrophils were detected by phase contrast microscopy (×200). b An orthographic microscope was used to detect the morphological characteristics of the neutrophils after Wright-Giemsa staining (×400). c Flow cytometry was performed to evaluate the surface characteristic antigens of the neutrophils. d Confocal microscopy was used to observe CD11b and CD33 antigens on the surface of the neutrophils (×600). e Laser scanning confocal microscopy was applied to identify the integrity of neutrophils labeled with DIO and DIL dyes (×600)