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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Biologically synthesized black ginger-selenium nanoparticle induces apoptosis and autophagy of AGS gastric cancer cells by suppressing the PI3K/Akt/mTOR signaling pathway

Fig. 5

KP-SeNPs stimulates autophagy in vitro. A LC3 immunofluorescence staining of AGS cells treated with KP-SeNPs (200 µg/mL) and rapamycin (5 µM). B Quantification of the LC3 puncta average area from the images using ImageJ software. Quantification was performed from 3 experiments with n > 20 cells for each condition. Autophagy-related C mRNA and D, E proteins in AGS cells treated with KP-SeNPs (150, 200 µg/mL) and KP-RE (150, 200 µg/mL) for 24 h; Rapa (rapamycin, 5 µM) was used as the positive control. F–H AGS cells were treated with KP-SeNPs (200 µg/mL); mRNA and proteins were collected at the indicated time points and analyzed by qRT-PCR and western blot, respectively. The western blotting images were quantified through Image J software. *p < 0.05, ** p < 0.01, ***p < 0.001 compared with control

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