Fig. 5
From: Milk-derived small extracellular vesicles: nanomaterials to promote bone formation
Milk-sEVs promoted osteoblast proliferation through AP3B1 transcription of GJA1. A The results of western blot; B the results of transfection of si-AP3B1 and si-USF2 into osteoblasts using RNA interference technology; C dual luciferase activity detected by co-transfection of Over-AP3B1 and plasmid GJA1-promoter, (+) indicated that Over-AP3B1 or GJA1-promoter was added, (−) indicates no addition; D we speculated that there were two specific binding sites for STAT5a on the GJA1 promoter; E the results of ChIP; F the results of agarose gel electrophoresis; G after addition of Milk-sEVs to si-AP3B1, western blotting showed that both AP3B1 and GJA1 expression increased. The statistical results are presented as means ± SD, ns: no significant difference. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001