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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Low-intensity pulsed ultrasound/nanomechanical force generators enhance osteogenesis of BMSCs through microfilaments and TRPM7

Fig. 3

A ALP staining and Alizarin Red S staining on day 7 and 21, respectively: macroscopic (top) and microscopic (bottom) images. B Quantitative assessment of staining indicators in (A). C WB analysis of the effect of each group on the level of osteogenic differentiation-related RUNX2, OPN, COLI, and TRPM7 proteins after 14 days of treatment. D Quantification of immunoreactive bands observed in WB analysis. E Immunofluorescence images stained with RUNX2 (green) and DAPI (blue). F Quantitative analysis of RUNX2 fluorescence intensity. G Micro-CT images of mouse calvarial bone regeneration. H–J Quantitative analysis of bone volume/total volume (BV/TV), trabecular number (Tb.N), and trabecular spacing (Tb.Sp) calculated based on micro-CT analysis (n = 6 per group). Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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