Fig. 7

A F-actin and G-actin levels in each group were assessed by WB and its quantitative analysis. B ALP staining and Alizarin Red S staining and related quantitative analysis were performed on day 7 and day 21, respectively. C The effect of each group on the level of osteogenic differentiation-related proteins (RUNX2, OPN, OCN, and TRPM7) after 14 days of treatment was assessed by WB and its quantitative analysis. D Immunofluorescence images stained with RUNX2 (green) and DAPI (blue) and related quantitative analysis. E Immunofluorescence images stained with TRPM7 (red), F-actin (green), and DAPI (blue). F Quantification of TRPM7 fluorescence intensity in panel E and actin fluorescence intensity along the white lines in panel E. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001