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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: ROS triggered local delivery of stealth exosomes to tumors for enhanced chemo/photodynamic therapy

Fig. 5

Efficient cell death induction by Stealth Exo@RB/Dox upon laser irrdatiation in vitro. A Schematic illustration of the procedure. A20 cells were treated with indicated exosomes and laser irradiation (532 nm laser, 0.1 W cm−2, 5 min). B Fluorescence images of ROS level analyzed by DCFH-DA staining in A20 cells with indicated treatments. Scale bar = 100 μm. C FCM analysis of cell death by Annexin V-APC/7-AAD double staining in cells treated same as above. D qPCR analysis of ferroptosis related genes in A20 cells treated as above. Gapdh served as internal controls. Data are expressed as mean ± SEM of three different experiments. *p < 0.05 by one-way ANOVA

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