Fig. 6

AMPK activation (AMPK phosphorylation) and HDAC inhibition activities in mice breast tumor tissues. A Timeline and treatment schedule for immunohistochemical staining of tumor tissues treated with free metformin, free OA-Met, free pro-DHA, free triptolide, HAOPs, HAOTs, DexOPTs and HAOPTs. B Images of breast tumor tissues stained by immunohistochemical staining with p-AMPK (brown). Scale bar, 100 µm. Quantitative analysis of C p-AMPK positive area and D Histone H3 (acetyl K9) positive area. Data were represented as mean ± SD (n = 4), *P < 0.05, **P < 0.01, ****P < 0.0001. E Images of breast tumor tissues stained by immunohistochemical staining with Histone H3 (acetyl K9) (brown). Scale bar, 100 µm