Fig. 4

The in vitro antimelanoma efficacy. A Cell viability of A375 cells treated with Exo, cRGD-Exo, TP solution, Exo/TP, and cRGD-Exo/TP. The concentrations of TP and Exo (protein) were both 70 ng/mL. B Anti-invasion effects on A375 cells of different groups via Transwell assay. (Scale bar = 50 μm). C Quantitation of cell invasion results. D Flow cytometry analysis of apoptosis. E Quantitative analysis of the cell apoptotic rate using ImageJ software. F The cell cycle distribution of different groups. G Quantitative analysis of the cell cycle distribution. *p < 0.05, **p < 0.01, ***p < 0.001