Fig. 6

Design, processing and architecture of the PCL/PLCL scaffold. A (a, b) The CT scanning images of the urethra; (d-f) The CT scanning images of the urethra filled with contrast reagent; (g) 2D slice (single layer) of the urethra design made using the WFIRM printing code program; where Red: UCs-laden hydrogel; Green: scaffold made of PCL/PLCL 50:50 blend; and Blue: SMCs-laden hydrogel. (h) The 3D rendering of the urethral design with porous scaffold and two hydrogel layers as seen using the WFIRM printing code program. B (a) PCL scaffold with columnar design; (b) PCL scaffold with spiral design; (c) PCL/PLCL (50:50) scaffold with columnar design; (d) PCL/PLCL (50:50) scaffold with spiral design; (e) native rabbit urethra. Scale bar: 2 mm (C) Stress testing of spiral and columnar scaffolds with PCL/PLCL blend (50:50). (D) UCs (labeled with green fluorescent dye) as seen in the hydrogel component of the bioprinted urethral construct after 1 day and 7 days of culture (a, c) and SMCs (labeled with red fluorescent dye) in the hydrogel component of the bioprinted urethral construct after 1 day of culture (b, d). Scale bar: 100 μm