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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Directing ricin-based immunotoxins with targeting affibodies and KDEL signal peptide to cancer cells effectively induces apoptosis and tumor suppression

Fig. 2

Flow cytometry and immunocytochemical analysis of cells treated with different RITs. A NIH3T6.7 and B SK-BR-3 cells were treated with PBS, RTA-KDEL, HER2Afb-RTA, or HER2Afb-KDEL. C MDA-MB-468 and D A431 cells were treated with PBS, RTA-KDEL, EGFRAfb-RTA, or EGFRAfb-RTA-KDEL. In each plot, the upper left and right quadrants represent necrosis and late apoptosis, respectively, and the lower left and right quadrants represent normal state of cells and early apoptosis, respectively. Relative populations of each quadrant are individually marked. Immunocytochemical detection of cleaved caspase-3 (red) and nuclei (blue) in: E NIH3T6.7 and F SK-BR-3 cells treated with HER2Afb-RTA or HER2Afb-RTA-KDEL; G MDA-MB-468 and H A431 cells treated with EGFRAfb-RTA or EGFRAfb-RTA-KDEL. Scale bars, 10 μm

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