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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Directing ricin-based immunotoxins with targeting affibodies and KDEL signal peptide to cancer cells effectively induces apoptosis and tumor suppression

Fig. 3

Fluorescence images of SK-BR-3 cells treated with A HER2Afb-RTA or C HER2Afb-RTA-KDEL at various time points. Nuclei, RTA, ER, and lysosomes are shown in blue, green, red, and white, respectively. Scale bars, 20 μm. One of the cells in A and C was selected and fluorescence intensity profiles of B HER2Afb-RTA and D HER2Afb-RTA-KDEL in the selected area were analyzed. The fluorescence intensity of RTA is represented by a colorimetric scale bar, and 2D projection of the area was drawn on top of the 3D plot. E Fluorescence signal intensity of HER2Afb-RTA or HER2Afb-RTA-KDEL bound to the cell surface at 0 h was statistically measured by line intensity analysis and plotted as a bar graph. All data are shown as means ± SD; ***p < 0.001. F Microvesicle-like structures containing RTA at the surface of SK-BR-3 cells treated with HER2Afb-RTA were enlarged with DIC images

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Journal of Nanobiotechnology

ISSN: 1477-3155

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