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Table 2 Yield and purity of genomic DNA extracted from S. aureus at various concentrations (10–104 CFU/mL) in the blood through preconcentration with van-SiO2-MNPs and van-PDA-MNPs

From: Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

CFU/mL

Van-SiO2-MNPs

Van-PDA-MNPs

Abs. (260/230)1

Abs. (260/280)2

DNA conc. (ng/µL)

Abs. (260/230)

Abs. (260/280)

DNA conc. (ng/µL)

10

1.9 ± 0.3

1.6 ± 0.2

17.1 ± 1.7

2.4 ± 0.1

1.9 ± 0.1

32.8 ± 0.6

102

1.9 ± 0.1

1.5 ± 0.7

6.4 ± 0.7

2.2 ± 0

1.8 ± 0

37.4 ± 0.3

103

1.8 ± 0

1.3 ± 0

10.9 ± 0.6

2.2 ± 0

1.6 ± 0.1

44.2 ± 1.2

104

2.1 ± 0.3

1.7 ± 0.3

18.6 ± 0.3

2.2 ± 0.1

1.8 ± 0.1

64.4 ± 0.6

  1. After preconcentration, bacterial DNA was extracted by using a kit (MagListo™ 5 M Genomic DNA extraction kit). DNA purity and concentration were determined by the ratio of absorbance (abs.) at 230, 260 and 280 nm by using a spectrophotometer (Nano-200)
  2. 1The absorbance ratio at 260 and 230 nm is used to assess the presence of contaminants such as phenol, that absorbs strongly at or near 230 nm
  3. 2The absorbance ratio at 260 and 280 nm is used to assess the presence of proteins and other contaminants that absorb strongly at or near 280 nm
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Journal of Nanobiotechnology

ISSN: 1477-3155

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