Fig. 4
(Reprinted with permission from Ref [56] Copyright © 2020, Cancers, Alekhya Mazumdar et al.)
Transcriptomic profiling of pre-metastatic lungs showed an inflammatory response and myeloid cell infiltration, which was promoted by sarcoma-derived extracellular vesicles (EVs). a Volcano plot of differentially expressed genes in pre-metastatic lungs. b Heatmap representation of up-regulated genes under “inflammatory response” in tumor-bearing mice and controls. Cut-off values of fold change are > 1.5 and FDR < 0.2. c Quantification of CD11b+ cells in lungs of controls and tumor-bearing mice (day 12 and day 28). d Mice were treated with either PBS, 143-B EVs, or SAOS-2 EVs (10 µg) 2 times a week for 3 weeks. e Representative immunofluorescence images of CD11b+ cells (red) and nuclei (blue) in lungs of treated mice. f Representative flow cytometric profile (left) and quantification (right) of CD11b+ cells isolated from lungs of treated mice