Fig. 4

A The cytotoxicity of various treatments towards A549 tumor cells. B Calcein AM/PI double stain of A549 cells after various treatments. Scale bar, 200 μm. C Fluorescence images of the cells stained by DCFDA to probe ROS generation after various treatments. Scale bar, 100 μm. D Fluorescence images of the cells stained by BODIPY-C11 to probe LPO accumulation after various treatments. Scale bar, 100 μm. E The effect of various ferroptosis inhibitors/inducers on cytotoxic activity of CH/DF (with laser) towards tumor cells. G The expression of HIF-1α protein after various treatments