Fig. 1

Construction and quality control of a synthetic nanobody library A. Sequence alignment of a camel nanobody (1ZVH) with the ASyNAL scaffold, showing the randomization regions in CDR1(27–33), CDR2(51–58) and CDR3(99–112) by X, respectively. The X is a mix of amino acids with the ratio shown below. B. NGS data (n = 20,112) showing the amino acid occurrence rate of ASyNAL at each position vs the designed rate. A total of 20,112 nanobody sequences were aligned, the amino acid residues at CDR regions were retrieved, and the rate of each amino acid occurrence (%) at each position was calculated