Fig. 5

Structural basis for the broad neutralization of C5G2. A, B Cryo-EM density maps of SARS-CoV-2 spike protein in complex with C5G2 viewed from the side A and top B, respectively. C5G2 is colored red, and the up RBD, down RBD, NTD, and glycans of the spike are colored teal, orange, blue and pink, respectively. The rest density of the spike is colored gray. C The 3.88 Å density map of local refinement of C5G2 and bound RBD and NTD. D Atomic model of C5G2:RBD:NTD. C5G2 is presented as a cartoon, and RBD and NTD are presented as surface representations. The epitopes of C5G2 on the RBD and NTD are both colored in teal. The decorated glycans on the RBD and NTD are shown as pink sticks. E, G Details of the interaction between C5G2 and the RBD E, F and NTD G. C5G2 is shown as a transparent cartoon, and the RBD and NTD are shown as a transparent surface. The residues involved in the hydrogen bond (gold dotted lines) interaction are shown as sticks. The contacts between C5G2 and NTD are also presented as green dotted lines. H RBD sequences of SARS-CoV-2 WT and its seven VOC variants (Alpha, Beta, Gamma, Delta, Omicron and its sublineages BA.2, BA.4/5) with a highlighted footprint of C5G2 (colored in light red)