Table 2 Anti-Spike nanobody affinity and neutralization potency

Spike

binding

KD(M)

3.05E-08

2.61E-07

1.46E-07

2.48E-07

Kon(1/Ms)

7.71E + 04

1.87E + 05

2.51E + 05

1.40E + 05

Kdis(1/s)

2.53E-03

4.87E-02

3.66E-02

3.46E-02

S1

binding

KD(M)

5.58E-10

Kon(1/Ms)

8.83E + 05

Kdis(1/s)

4.93E-04

RBD

binding

KD(M)

2.66E-07

1.80E-08

1.62E-09

3.48E-09

Kon(1/Ms)

2.59E + 05

1.44E + 04

1.76E + 04

1.63 + 04

Kdis(1/s)

6.87E-02

2.59E-04

2.84E-05

5.66E-05

Pseudo-

virus

IC₅₀(M)

WT

N.E.

N.E.

1.59E-09

1.37E-08

Alpha

N.E.

N.E.

9.5E-10

2.69E-08

Beta

N.E.

8.16E-08

5.6E-10

N.E.

Gamma

N.E.

N.E.

1.06E-09

N.E.

Delta

N.E.

N.E.

N.E.

2.05E-08

Omicron

N.E.

2.1E-09

3.0E-10

N.E.