Fig. 4

Intracellular distribution and ion regulation of AINR in 4T1 cells. A CLSM images and co-localization analysis of 4T1 cells after treatment with RB loaded polymer for different time. RB (red color) was used to mark F68@TA polymer, and lyso marker (green color) was used to indicate lysosomes. B Fluorescence images of 4T1 cells treated with different concentration of CDDP for 18 h. After treatment, cells were stained with H₂O₂ green probe (50 μΜ) for 30 min at 37 ℃. C Biological electron microscopy images of 4T1 incubated with F68@TA polymer, F68@TA@Ag and AINR for 5 h. Black arrow: polymer, blue arrow: F68@TA@Ag, and red arrow: F68@TA@Ag after dissolution of Ag NPs. D CLSM images of 4T1 cells stained by MQAE probe (5 mM for 1 h at 37 ℃) to mark intracellular Cl⁻ after treatment with CDDP, polymer, F68@TA@Ag and AINR for 18 h. Excitation wavelength: 405 nm and Emission wavelength: 420–470 nm. E CLSM images of 4T1 cells stained by Fe²⁺ probe (1 μM for 30 min at 37 ℃) to mark intracellular free Fe²⁺ after treatment with CDDP, F68@TA, F68@TA@Ag and AINR for 18 h. Excitation wavelength: 552 nm and Emission wavelength: 570–620 nm