Fig. 6

Movability and deep penetration ability of F68@TA@Ag in 3D MCS and tumor tissues. A Schematic diagram of investigation on deep penetration capacity in 3D MCS. MCS was constructed on low melting point agarose. Prior to the treatment of RB (10 μg/mL) labeled F68@TA or F68@TA@Ag, 3D MCS was incubated with 100 μM H₂O₂ was added for 12 h. CLSM was used to observe RB distribution at different focal planes. B, C Fluorescence images of MCS in different focal planes after 4T1 cells were incubated with B F68@TA/RB and C F68@TA@Ag/RB for 12 h. Scale bar: 75 μm. D Schematic diagram of investigation on deep penetration capacity in tumor tissues. The movement mechanism was that asymmetrical Ag NPs on F68@TA@Ag could react with H₂O₂ at tumor acid environment, leading to O₂ generation. The reaction equation was presented above. E, F Immunofluorescent staining of tumor tissues from E F68@TA/RB and F F68@TA@Ag/RB group. Red color represented nanoparticles and green color represented tumor vessels