Fig. 7

Anti-tumor efficacy of AINR in vivo. A Biodistribution of F68@TA@Ag/IR783 in 4T1-bearing BALB/C mice collected by IVIS imaging system at different time point. The red circles referred to tumor tissues. B Optical images of visceral organs exfoliated after injection for 48 h. The groups in A-B were a) IR783 and b) F68@TA@Ag/IR783. C Schematic diagram of treatment program. D The changes of tumor volume with different treatments (n = 5). E Representative Cl⁻ staining of tumor tissues from 4T1-bearing BALB/C mice receiving different treatments. F Representative Fe²⁺ staining of tumor tissues from 4T1-bearing BALB/C mice receiving different treatments. G H&E staining and TUNEL staining of tumor tissues from 4T1-bearing BALB/C mice receiving different treatments, the circle represents nuclear loss, the arrow represents karyopyknosis. In all experiments, concentration of CDDP was set as 2 mg/kg, and statistical analysis was performed by one-way ANOVA analysis. *p < 0.05, **p < 0.01, ***p < 0.001