Fig. 2
Tumor targeting ability of Rg3-Lp. Molecular docking of Glut1 with Rg3 (yellow sticks) (A) and cholesterol (blue sticks) (B), respectively. H-bond interactions between Rg3 and Glut1 were represented by the yellow dotted lines. C The quantitative analysis of cellular uptake of C-Lp/C6, Rg3-Lp/C6 and Rg3-Lp/C6 with Glut1 inhibitors in 4T1 cells via flow cytometry. The fluorescent intensity represents the mean fluorescence intensity (MFI) of C6-loaded liposome uptake by 4T1 cells. D Flow cytometry analysis of cellular uptake of C-Lp/C6 and Rg3-Lp/C6 in normal and 4T1Glut1− cells, respectively. The fluorescent intensity represents the mean fluorescence intensity (MFI) of C6-loaded liposome uptake by 4T1 cells. E Representative confocal laser scanning microscope (CLSM) images of cellular uptake of C-Lp/C6 and Rg3-Lp/C6 in 4T1 cells before and after Glut1 knockdown. Blue: cell nucleus; green: liposomes; red: Glut1. Scale bar, 10 µm. F Biodistribution of the DID-labeled liposomes in 4T1 tumor-bearing mice at different time points after intravenous injection. G Ex vivo imaging of dissected tumors 24 h after injection of C-Lp/DiD and Rg3-Lp/DiD, respectively. H Semi-quantitative ROI values of mean fluorescence intensity at tumor sites. **p < 0.01; Data are shown as mean ± standard deviation of three technical replicates; Differences between two groups were analyzed with unpaired t-test, one-way ANOVA was performed to compare data among multiple groups