Fig. 7
Analysis of immune cells in TME. A Flow cytometric and histogram analysis of the relative abundance of CD4+ or CD8+ T cells over total lymphocyte cells (CD45+ cells) in tumors treated with PBS, DTX, C-Lp/DTX, Nanoxel-PM, Rg3, Rg3-Lp, Rg3/DTX and Rg3-Lp/DTX, respectively. B The relative abundance of CD86 positive M1-type and CD206 positive M2-type macrophages over total macrophages populations (CD45+F4/80+) in tumors treated with different groups. C Flow cytometric and histogram analysis of the relative abundance of CD11b+/Gr-1+ MDSC cells over total lymphocyte cells (CD45+ cells) in tumors treated with different groups, including Gr-1highCD11b+ granulocytic (G-MDSC) and Gr-1intCD11b+ monocytic (M-MDSC) MDSC. D Flow cytometry gating and histogram analysis of the relative abundance of CD4+FoxP3+ Treg cells over total lymphocyte cells (CD45+ cells) in tumors. *P < 0.05, **P < 0.01, and ***P < 0.001; One-way ANOVA was performed to compare data among multiple groups; Error bars represent mean ± SD of three independent experiments